The detection antibody is incubated for 1. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Make sure the setup for Eu Cryptate is used. 4. For more. Plate 25 μL of cells in. Read & analyse. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. For more information about HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. This work reports 25 small-molecule binders of the m 6 A-reader domain of YTHDC1. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. For more information. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. This protocol gives the cells viability and confluence to be monitored. 96-well culture plateFOR HTRF CERTIFIED READER. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Incubate overnight at RT. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. PerkinElmer EnVision Multimode Plate Reader. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. Plus . S. Assay setup for a 384-well low-volume plate. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Manufacturer. Make sure the setup for Tb Cryptate is used. For more information about HTRF ® compatible readers and for set-up recommendations, please. SV detection plate. These plates can be read on all HTRF certified readers, thus making their implementation in. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Excitation lasers significantly improve performance and lower. Read & analyse. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The signals are produced by, converted by, or transmitted through the. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The optional HTRF-certified filter-based module may be. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. These plates can be read on all HTRF certified readers, thus making their implementation in. 1. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. Neocarzinostatin dose-response on Jurkat cells. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. For a list of HTRF-compatible readers and set-up recommendations, please visit . com HTRF Microplate Readers. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. FOR HTRF CERTIFIED. Figure 1. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. protocol with suspension cells or with a. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Combined with our Kaleido™ workflow software, it. In this case the Z´(pronounced Z prime) value comes to mind. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. Figure 3. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. DATA REDUCTION & INTERPRETATION 1. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The ratio for each well must be calculated individually. The VICTOR Nivo. 10 µL Standard or Sample. * For HTRF microplate recommendations, please visit . PerkinElmer. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Make sure the setup for Tb Cryptate is used. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. 5 EXAMPLE OF MAP PLATE . + 4 µL HTRF pre-mixed antibodies. But sometimes these parameters are not sufficient enough. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Make sure the setup for Eu 3+ Cryptate is used. FOR HTRF CERTIFIED READER. The PHERAstar ® FSX high-throughput screening microplate reader uses unique, easy-to-configure optic modules. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These are the different kinetic modes on BMG LABTECH microplate readers. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Make sure the setup for Tb Cryptate is used. This protocol, HTS designed, allows miniaturization while. Remove the plate seal and read on an HTRF compatible reader. When MikroWin opens, if you already have a pre-existing template for. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. For more information. For more information. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. → Cover the plate with a plate sealer. At that stage, the analyte is sandwiched between the 2 antibodies. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. To perform the assay, use white plate only. Even if not listed, some. 3. No washing steps are required. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. Incubate 4h at RT. FOR HTRF CERTIFIED READER. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. + 4 µL HTRF pre-mixed antibodies . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Alpha Technology: The VICTOR Nivo system. For more information. Simply place the module into one of the five module bays and read the assay. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5. Handle complex biological matrices easily. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate was covered and incubated for one hour at room temperature. It is typically used to determine the concentration of nucleic acids and proteins. 5 µL. 15 / Each of 1; Qty. This protocol gives the cells viability and confluence to be monitored. These plates can be read on all HTRF certified readers, thus making their implementation in. Choosing the right microplate reader ensures you’ll get an optimal readout. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Incubate 24 hours at RT. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It features a modular design that allows users to add or change detection cartridges according to their needs. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Transcreener® ADP2 TR-FRET assay. + 4 µL HTRF pre-mixed antibodies . The new Molecular Devices instrument comes with a standard 1-year depot warranty service. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. Incubate overnight at RT. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. These plates can be read on all HTRF certified readers, thus making their implementation in. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF plate reader. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 μL of Binding buffer 2X. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. • HTRF®-Certified Reader. For more ® information about HTRF compatible readers and for set-up recommendations. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. Discover our high performance reader. The plate reader. FOR HTRF CERTIFIED READER For more. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Introduction. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). It also integrates with the. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. SERIAL DILUTIONS . FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF-compatible SpectraMax Multi-Mode Readers. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). For more information. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in. CLARIOstar is equipped with a specific optical device which enables the measurement of. Read & analyse. It can also be further streamlined to a one-. 2. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Microplate Readers and Reader Accessories. Read & analyse. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Read & analyse. It continues to deliver optimal results. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Push the screw down and turn it clockwise to lock the plate. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. FOR HTRF CERTIFIED. Eu cryptate emission level). The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. View Brochure. If not, our scientific team will help you choose the instrument that best suits your needs and budget. 1. Incubate 4h at RT. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. PURCHASE SEPARATELY . Remove the plate sealer and read on an HTRF® compatible reader . As shown in Fig. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Make sure the setup for Eu 3+ Cryptate is used. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. No washing steps are required. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. FOR HTRF CERTIFIED. In particular, these parameters should be entered as below. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF is compatible with a large range of available microplate readers. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Customer Transformation Hub. For more information about HTRF. 0. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Catalog No. For more information about HTRF. The assay was prepared in both white and black plates and incubated >18 hours. It can also be further streamlinedFOR HTRF CERTIFIED READER. Incubate overnight at RT. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Specialized filters allow for IR fluorescence. This protocol enables the cells' viability and confluence to be monitored. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. Revvity. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. SpectraMax i3/i3x and Paradigm readers can be equipped with. The ratio between control lysate signal / non-specific signal should be greater than 2. FOR HTRF CERTIFIED READER. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Fluorescence intensity – Fluorescence intensity is one of the most. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER For. For more information about HTRF ®• HTRF®-Certified Reader. The plate was covered and incubated for two hours at room temperature. Read & analyse. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. If not, our scientific team will help you choose the instrument that best suits your needs and budget. 5 hours before a further 3 washing steps. The VICTOR Nivo is a HTRF certified microplate reader. Read & analyse. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. best fluorescence polarization plate reader on the market. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. • HTRF®-Certified Reader. Table 1. Highly compatible plates for HTRF. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. It accepts all types of microtitration plate. No washing steps are required. Highly compatible plates for HTRF. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The optimized well design and high quality plastics provides low background and crosstalk. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Plate 25 μL of cells in. This protocol. Plate cells Stimulate cells 3. For more. Up to 8 decades dynamic range in one measurement with EDR technology. It can also be further streamlined toFOR HTRF CERTIFIED READER. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 1 – 1. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. HTRF Setup. 3-in-1 absorbance using a variety of sample formats, including microplates. Incubate overnight at RT. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. PROTOCOL AT A GLANCE. Make sure the setup for Tb Cryptate is used. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. For the standard curve described, all wells of the microplate are read. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate overnight at RT. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. HTRF-specific. FOR HTRF CERTIFIED READER. • HTRF®-Certified Reader **. 14 seconds for a 384-well plate. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. HTRF Signal + 5 hours @ RT or . FOR HTRF CERTIFIED READER. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. FOR HTRF CERTIFIED. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. Assay setup for a 384-well low-volume plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Read other about this measurement approach here. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. Typical applications The HTRF cartridge provides optimal performance for key applications. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. As shown in Fig. $1,024. COMPONENTS ; STORAGE . Each well requires 10 μL of 4X lysis buffer /well. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. FOR HTRF CERTIFIED READER. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. HTRF-specific. FOR HTRF CERTIFIED READER For more information about HTRF. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. FlexStation® 3 Multi-Mode Microplate Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . FOR HTRF CERTIFIED READER. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. This protocol gives the cells viability and confluence to be monitored. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 41 mg/ml) was obtained from Thermo Fisher Scientific. Make sure the setup for Eu3+ Cryptate is used. FOR HTRF CERTIFIED READER For more. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. • HTRF®-Certified Reader. FOR HTRF CERTIFIED READER. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. • HTRF®-Certified Reader.